Scaffolds

Crosslinked gelatin nanofibers

Artificial basement membrane

Electrospun gelatin nanofibers deposited onto lithographically patterned hexagonal frames and subsequently chemically crosslinked can serve as versatile scaffolds with minimum exogenous materials for both cell and organoid culture. They can also be used for molecular self-assembly and in particular network forming of basement membrane proteins, resulting in in vivo like basement membrane structures for organ-on-chip applications. Different monolayer nanofiber scaffolds can also be engineered to further recapitulate the in vivo architecture and biochemical complexity of native extracellular matrix.